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Brief description

The specified quantity calculated on each row/column is subtracted from each value.

Output: Normalized expression matrix.


Matrix access

Specifies whether the subtraction is performed on rows or columns (default: Rows). If the subtraction is performed on rows, the “Grouping” can be specified as well (default: <No grouping>).


This parameter is just relevant, if the “Matrix access” is set to “Rows”. It specifies, whether the normalization should be applied separately on groups (default: <No grouping>).

Subtract what

Defines what value should be subtracted from all entries in expression columns (default: Median). The subtrahend can be selected from a predefined list:

  • Mean
  • Median
  • Most frequent value
  • Tukey's biweight

Parameter window

Perseus pop-up window: Normalization -> Subtract

perseus/user/activities/matrixprocessing/normalization/subtract.txt · Last modified: 2015/12/09 16:28 by temu