The specified quantity calculated on each row/column is subtracted from each value.
Output: Normalized expression matrix.
Specifies whether the subtraction is performed on rows or columns (default: Rows). If the subtraction is performed on rows, the “Grouping” can be specified as well (default: <No grouping>).
This parameter is just relevant, if the “Matrix access” is set to “Rows”. It specifies, whether the normalization should be applied separately on groups (default: <No grouping>).
Defines what value should be subtracted from all entries in expression columns (default: Median). The subtrahend can be selected from a predefined list: